Home Page Researchers Aaron
School of Computer Science and Engineering, Faculty of Science
The Hebrew University, Jerusalem 91904, Israel
Tel: +972-2-6584764; Fax: +972-2-6798074
Nano Optics & NanoMedicine/Nanochemistry
Our work during the past year has concentrated on two topics that have led to publications
The first paper addresses the inverse problem of optical imaging and the dream of addressing both 3D structure (phase) as seen in atomic force microscopy and super-res0lution using parallel imaging. In a paper published in ACS Nano listed below the first of these problems have been solved exactly and we are in the process of addressing the second aspect of the problem as noted above. In order to appreciate our advance one notes that an object is characterized by its amplitude and phase. However, when acquiring optical data about such an object, using a recording medium such as a camera, phase information is lost. Crystallography experienced a breakthrough in phase retrieval for large molecular entities by Max Perutz's introduction of ?heavy atoms? using the method of isomorphous replacement. The availability of scanning probe microscopy and its full integration with optical microscopy allows us to apply these X-ray concepts to implement ?heavy atom? restoration of phase in optical phase retrieval. In analogy to the heavy atom method, we acquire Fourier intensities in place of an X-ray diffraction pattern, and in place of the heavy atom, we utilize a nanometrically translatable point source of light based on the propagating field of a cantilevered near-field scanning optical
microscopic (NSOM) probe controlled by an atomic force microscope (AFM). This integration of NSOM/AFM technology with far-field imaging achieves robust phase retrieval independent of external parameters, leading to 3D optical imaging. The methodology has super-resolution potential, and thus, heavy atom restoration of phase with super-resolution (HARPS) shows the potential of transparently integrated scanning probe microscopy with optical and other imaging modalities such as electron or ion optical imaging.
A second paper records an advance we have made in NanoMedicine/Nanochemistry. It uses advances in the ultrafast manipulation of light to address a general need in medicine for a clinical approach that can provide a solution to a variety of disorders requiring subsurface tissue manipulation with ultralow collateral damage. Examples are age-related macular degeneration (AMD), fungal infections, tumors surrounded by overlying tissue, cataracts, etc. Although lasers have revolutionized the use of light in clinical settings, most lasers employed in medicine cannot address such problems of depth-selective tissue manipulation. This arises from the fact that they are mostly based on one photon based laser tissue interactions that provide a cone of excitation where the energy density is sufficiently high to excite heat or fluorescence in the entire cone. Thus, it is difficult to excite a specific depth of a tissue without affecting the overlying surface. However, the advent of femtosecond (fs) lasers has caused a revolution in multiphoton microscopy (Zipfel et al. Nat. Biotechnol. 2003, 21, 1369?1377; Denk et al. Science1990, 248, 73?76) and fabrication (Kawata et al. Nature 2001, 412, 697?698). With such lasers, the photon energy density is only high enough for multiphoton processes in the focal volume, and this opens a new direction to address subsurface tissue manipulation. Here we show in an AMD animal model, Ccr2 KO knockout mutant mice, noninvasive, selective fs two-photon photobleaching of pigments associated with AMD that accumulate under and in ultraclose proximity to the overlying retina. Pathological evidence is presented that indicates the lack of collateral damage to the overlying retina or other surrounding tissue.
Specific research topics related to Nanoscience and Nanotechnology:
- Nano Optics
- Probing Electromagnetic Waves in Photonic, Plasmonic and Silicon Based Device Structures with Sub-wavelength Resolution
- Zeptoliter nanochemical measurments of ionic concentration above and at living cell membranes using Near-field Scanning Optical Microscopy
- Fountain Pen Nanochemical Writing
- Atomic Force Controlled Electrophoretic Deposition for protein arrays for protein protein interaction sensing
- NanoRaman/MultiProbe AFM of Strained Silicon with Structural & Functional Nanometric Imaging
- Non-linear optical functional imaging
List of publications in nanoscience and nanotechnology (2008-2011)
- Danielle R. Honigstein, Jacques Weinroth, Michael Werman, and Aaron Lewis, ?Noniterative Exact Solution to the Phase Problem in Optical Imaging Implemented with Scanning Probe Microscopy,? ACS Nano doi.org/10.1021/nn203427z (2012)
- Zakhariya Manevitch, Aaron Lewis, Carol Levy, Evelyne Zeira, Elina Ovin, Eyal Banin, Alexandra Manevitch, Artium Khatchatouriants, Jacob Pe?er, Eithan Galun, Itzhak Hemo, ?Subsurface Femtosecond Tissue Alteration with Ultralow Collateral Damage: Selective Photobleaching of Macular Degeneration Pigments in Near Retinal Contact? J. Phys. Chem. B 116, 6945?6951 (2012) [dx.doi.org/10.1021/jp300355m]
- Special Issue Materials Science and Engineering on Advanced Functional Materials, 143, 921 ( 2008); Editors: S. Maier, B,. Barnes, S. Huant, A. Lewis and E. Ozbay.
- Dmitry Lev, Alexandra Manevich, Zacharia Manevich, Alexander Puzenko, Leonid Livshits, Yuri Feldman, and Aaron Lewis, "D-Glucose-Induced Second Harmonic Generation Response in Human Erythrocytes," J. Physical Chemistry B 113, 2513 (2009)
- Zacharia Manevitch, Dmitry Lev, Malka Hochberg, Mila Palhan, David Enk & Aaron Lewis, "Selective Elimination of Fungal Infections Within and Below Surfaces Using a Femtosecond Laser," Photochemistry and Photobiology, 86, 476-479 (2010)
- Leslie M. Loew and Aaron Lewis, " Second Harmonic Imaging of Membrane Potential," Dejan Zecevic, Yale Physiology Editor, Springer Verlag Neuroscience Series (in press 2009)
- Yulia Lovsky & Aaron Lewis & Chaim Sukenik & Eli Grushka, "Atomic-force-controlled capillary electrophoretic nanoprinting of proteins," Anal Bioanal Chem DOI 10.1007/s00216-009-3089-2009
- P. Theer, W. Denk, M. Sheves, A. Lewis and P. B. Detwiler, "," Biophys J. 100, :232-42 (2011)
Danielle Honigstein, Jacques Weinroth, Michael Werman and Aaron Lewis, Applying Heavy Atom Concepts With Scanning Probe Technology To Achieve A Non-Iterative Exact Solution to the Phase Problem in Optical Imaging (in press ACS Nano)
Cooperation with other researchers/universities:
Within Hebrew University:
- Hermona Soreq, Hebrew University, Life Sciences
- Michael Werman, Hebrew University, Computer Sciences
With other universities:
- Professor Chaim Sukenik - Department of Chemistry, Bar Ilan University
- Stefan Maier, Imperial College London
- Eleanor Campbell, University of Edinburough
- Stefanie Reich, Technical University of Berlin.
Students, postdocs and researchers:
Ph.D. students:Talia Yeshua; Danielle Honigtein; Nataly Cohen, Dimitry Lev; Aharon Brahami; Avi Israel; Yakov Weinroth.
M.Sc students: Daniel Sabag; Anis Bishara, Hadas Levy.
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